Hotspot

psbM-trnD-GUC

LSC · 31293-32343 · 1051 bp

Back to Fontanesia

Pi 0.0115

MarkerSeek score 59.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1051
variable_sites: 12
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.4	58.5	1183-1192 bp	1.0	56.4
psbM-trnD-GUC_p2	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.4	58.5	1175-1184 bp	1.0	56.2
psbM-trnD-GUC_p3	AAGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1176-1185 bp	1.0	54.7
psbM-trnD-GUC_p4	AGCGGTAGGAACTAGAATGAA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1175-1184 bp	1.0	54.7
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.1	58.5	1184-1193 bp	1.0	54.2