Hotspot

atpH-atpI

LSC · 14623-15735 · 1113 bp

Back to Fontanesia

Pi 0.00809

MarkerSeek score 63.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1113
variable_sites: 9
pi: 0.00809
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1248-1249 bp	1.0	78.3
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TCCCTGTCATGTTCCTTGGA	55.0	50.0	58.1	58.3	1331-1332 bp	1.0	77.4
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TCCCTGTCATGTTCCTTGGA	55.0	50.0	57.9	58.3	1335-1336 bp	1.0	77.4
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.2	58.3	1249-1250 bp	1.0	77.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1263-1264 bp	1.0	76.8