Hotspot

trnH-GUG-psbA

LSC · 94-543 · 450 bp

Back to Fontanesia

Pi 0.0671

MarkerSeek score 75.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 29
pi: 0.0671
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTAGTTATGGGCGAACGACG	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	58.1	58.0	1202-1206 bp	1.0	82.9
trnH-GUG-psbA_p2	TTAGTTATGGGCGAACGACG	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	58.1	58.0	1208-1212 bp	1.0	82.9
trnH-GUG-psbA_p3	TTAGTTATGGGCGAACGACG	GGAGCTTAGTTTCCGTCTGG	50.0	55.0	58.1	58.0	1203-1207 bp	1.0	82.9
trnH-GUG-psbA_p4	TTAGTTATGGGCGAACGACG	GGGAGCTTAGTTTCCGTCTG	50.0	55.0	58.1	58.0	1204-1208 bp	1.0	82.9
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	1147-1151 bp	1.0	82.7