Hotspot

accD

LSC · 64466-66667 · 2202 bp

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Pi 0.0154
MarkerSeek score 49.90
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
2202
variable_sites
25
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.74
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 GCAGTTTTCCTGAATCAAGCC CCATTGCAATTGCCGGAAAT 47.6 45.0 58.0 58.0 2406-2981 bp 1.0 76.3
accD_p2 TGATACGACAAAAGCGCTCT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.6 58.0 2819 bp 0.5 56.9
accD_p3 TGATACGACAAAAGCGCTCT AAGAAGCCATTGCAATTGCC 45.0 45.0 57.6 57.9 2825 bp 0.5 56.3
accD_p4 TGATACGACAAAAGCGCTCT GCCGGAAATACTAGGCCTAC 45.0 55.0 57.6 57.5 2808 bp 0.5 53.5
accD_p5 TGATACGACAAAAGCGCTCT GGTCCATCGGGTCTAAACAA 45.0 50.0 57.6 57.2 2872 bp 0.5 50.9