Hotspot

ndhG-ndhI

SSC · 126199-126369 · 171 bp

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Pi 0.0491
MarkerSeek score 75.70
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
171
variable_sites
8
pi
0.0491
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 CCCTATCATTCAATATCTGGAA GCACAATTTCGAATTCGCCT 36.4 45.0 52.8 57.7 274-276 bp 1.0 44.7
ndhG-ndhI_p2 CCCTATCATTCAATATCTGGAAA GCACAATTTCGAATTCGCCT 34.8 45.0 53.7 57.7 274-276 bp 1.0 44.7
ndhG-ndhI_p3 CCCTATCATTCAATATCTGGAA TCGCACAATTTCGAATTCGC 36.4 45.0 52.8 58.1 276-278 bp 1.0 44.6
ndhG-ndhI_p4 CCCTATCATTCAATATCTGGAAA TCGCACAATTTCGAATTCGC 34.8 45.0 53.7 58.1 276-278 bp 1.0 44.6
ndhG-ndhI_p5 CCCTATCATTCAATATCTGGAA TCAAATTGCTTTGGGTCGGT 36.4 45.0 52.8 58.3 327-329 bp 1.0 43.9