Hotspot

rpl32-trnL-UAG

SSC · 120711-121531 · 821 bp

Back to Fockea

Pi 0.0183
MarkerSeek score 69.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
821
variable_sites
15
pi
0.0183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGAAGGGATATTGGACAGCA TTAGAAGATGACATGCCGCC 50.0 50.0 57.0 58.0 1009-1014 bp 1.0 72.7
rpl32-trnL-UAG_p2 GGAAGGGATATTGGACAGCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.0 57.9 955-960 bp 1.0 72.1
rpl32-trnL-UAG_p3 GGAAGGGATATTGGACAGCA AGCAGCGTGTCTACCAATTT 50.0 45.0 57.0 57.8 948-953 bp 1.0 71.6
rpl32-trnL-UAG_p4 GGAAGGGATATTGGACAGCA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.0 57.8 954-959 bp 1.0 71.5
rpl32-trnL-UAG_p5 GGAAGGGATATTGGACAGCA CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.0 57.8 953-958 bp 1.0 71.5