Hotspot

ycf4-cemA

LSC · 68060-68737 · 678 bp

Pi 0.0134

MarkerSeek score 66.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 9
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAAGCCACCAAGG	45.0	55.0	58.1	57.9	780-785 bp	1.0	79.9
ycf4-cemA_p2	ACAAAAAGCTGCGGAATTGG	GAGAGAGAAAGCCACCAAGG	45.0	55.0	57.8	57.9	796-801 bp	1.0	79.4
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CCGGCATTCCACCAATTAGT	45.0	50.0	58.1	58.2	828-833 bp	1.0	78.8
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	AGAGAGAAAGCCACCAAGGA	45.0	50.0	58.1	58.3	779-784 bp	1.0	78.6
ycf4-cemA_p5	ACAAAAAGCTGCGGAATTGG	CCGGCATTCCACCAATTAGT	45.0	50.0	57.8	58.2	844-849 bp	1.0	78.3