Hotspot

rps2-rpoC2

LSC · 16430-21908 · 5479 bp

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Pi 0.00973
MarkerSeek score 43.80
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
5479
variable_sites
53
pi
0.00973
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 GCCTTCGATGGTTACGTCTT CGGCATTCTGTTCTACAGCT 50.0 50.0 58.0 58.0 674-699 bp 1.0 84.6
rps2-rpoC2_p2 GCCTTCGATGGTTACGTCTT AAAGGTGAACGGCATTCTGT 50.0 45.0 58.0 58.0 683-708 bp 1.0 84.5
rps2-rpoC2_p3 GCCTTCGATGGTTACGTCTT AAGGTGAACGGCATTCTGTT 50.0 45.0 58.0 58.0 682-707 bp 1.0 84.5
rps2-rpoC2_p4 GCCTTCGATGGTTACGTCTT CTGAGGAAGCCCTTTACGAC 50.0 55.0 58.0 58.0 770-795 bp 1.0 84.1
rps2-rpoC2_p5 GCCTTCGATGGTTACGTCTT ATCACCATCTTGACCGCTTC 50.0 50.0 58.0 58.0 1317-1342 bp 1.0 82.5