primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	76	350	344-344	1.000000	83.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	76	350	391-391	1.000000	83.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	76	350	405-405	1.000000	83.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	76	350	404-404	1.000000	83.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	76	350	406-406	1.000000	83.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6107	7337	1304-1327	1.000000	79.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6107	7337	1315-1338	1.000000	79.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6107	7337	1308-1331	1.000000	74.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6107	7337	1334-1357	1.000000	74.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	6107	7337	1316-1339	1.000000	74.5
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9729	9907	254-259	1.000000	66.2
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9729	9907	257-262	1.000000	65.9
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9729	9907	260-265	1.000000	65.4
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9729	9907	263-268	1.000000	65.2
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9729	9907	258-263	1.000000	63.7
rps2-rpoC2_p1	rps2-rpoC2	1	GCCTTCGATGGTTACGTCTT	CGGCATTCTGTTCTACAGCT	20	20	50.0	50.0	58.0	58.0	17.8	2.0	0.021706	20774	20893	674-699	1.000000	84.6
rps2-rpoC2_p2	rps2-rpoC2	2	GCCTTCGATGGTTACGTCTT	AAAGGTGAACGGCATTCTGT	20	20	50.0	45.0	58.0	58.0	17.8	0.0	0.024714	20774	20893	683-708	1.000000	84.5
rps2-rpoC2_p3	rps2-rpoC2	3	GCCTTCGATGGTTACGTCTT	AAGGTGAACGGCATTCTGTT	20	20	50.0	45.0	58.0	58.0	17.8	0.0	0.024714	20774	20893	682-707	1.000000	84.5
rps2-rpoC2_p4	rps2-rpoC2	4	GCCTTCGATGGTTACGTCTT	CTGAGGAAGCCCTTTACGAC	20	20	50.0	55.0	58.0	58.0	17.8	0.0	0.017511	20774	20893	770-795	1.000000	84.1
rps2-rpoC2_p5	rps2-rpoC2	5	GCCTTCGATGGTTACGTCTT	ATCACCATCTTGACCGCTTC	20	20	50.0	50.0	58.0	58.0	17.8	0.0	0.035809	20774	20893	1317-1342	1.000000	82.5
petN-psbM_p1	petN-psbM	1	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	34521	35714	1288-1288	1.000000	72.3
petN-psbM_p2	petN-psbM	2	TTTCCCTTTCGCTCGTAGTG	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.102641	34521	35714	1300-1300	1.000000	71.7
petN-psbM_p3	petN-psbM	3	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	34521	35714	1284-1284	1.000000	71.7
petN-psbM_p4	petN-psbM	4	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.108380	34521	35714	1287-1287	1.000000	71.7
petN-psbM_p5	petN-psbM	5	ACATTTTCCCTTTCGCTCGT	TCATTCTAGTTCCTACCGCCT	20	21	45.0	47.6	58.1	58.0	0.0	0.0	1.135885	34521	35714	1304-1304	1.000000	71.5
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGATGT	ATCAAGCTGCCGAGTATTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.085081	48255	49131	1027-1055	1.000000	82.1
psaA-ycf3_p2	psaA-ycf3	2	TGAGAAATGACCCGGTTTGG	ATCAAGCTGCCGAGTATTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136672	48255	49131	1046-1074	1.000000	81.6
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGATGT	CGATCAAGCTGCCGAGTATT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.186031	48255	49131	1029-1057	1.000000	81.3
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGATGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.207941	48255	49131	956-984	1.000000	81.3
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGATGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.235712	48255	49131	960-988	1.000000	81.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGTTAATCGGGGCT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.321560	57170	58645	1546-1621	1.000000	82.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	57170	58645	1532-1607	1.000000	82.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GTCCTTTGTTAATCGGGGCT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.0	0.0	0.665332	57170	58645	1545-1620	1.000000	79.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	5.2	0.0	0.680379	57170	58645	1531-1606	1.000000	79.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGTCCTTTGTTAATCGGGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.697675	57170	58645	1548-1623	1.000000	79.2
accD_p1	accD	1	GCAGTTTTCCTGAATCAAGCC	CCATTGCAATTGCCGGAAAT	21	20	47.6	45.0	58.0	58.0	0.0	26.9	1.075277	64466	66667	2406-2981	1.000000	76.3
accD_p2	accD	2	TGATACGACAAAAGCGCTCT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	12.1	26.9	0.390791	64466	66667	2819-2819	0.500000	56.9
accD_p3	accD	3	TGATACGACAAAAGCGCTCT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	57.6	57.9	12.1	26.8	0.467325	64466	66667	2825-2825	0.500000	56.3
accD_p4	accD	4	TGATACGACAAAAGCGCTCT	GCCGGAAATACTAGGCCTAC	20	20	45.0	55.0	57.6	57.5	12.1	8.9	0.816083	64466	66667	2808-2808	0.500000	53.5
accD_p5	accD	5	TGATACGACAAAAGCGCTCT	GGTCCATCGGGTCTAAACAA	20	20	45.0	50.0	57.6	57.2	12.1	0.0	1.137780	64466	66667	2872-2872	0.500000	50.9
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	58.1	57.9	3.3	0.0	0.202190	68060	68737	780-785	1.000000	79.9
ycf4-cemA_p2	ycf4-cemA	2	ACAAAAAGCTGCGGAATTGG	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	57.8	57.9	0.0	0.0	0.259844	68060	68737	796-801	1.000000	79.4
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	CCGGCATTCCACCAATTAGT	20	20	45.0	50.0	58.1	58.2	3.3	0.0	0.333006	68060	68737	828-833	1.000000	78.8
ycf4-cemA_p4	ycf4-cemA	4	TTGGCCTATTTCTTGCGTGT	AGAGAGAAAGCCACCAAGGA	20	20	45.0	50.0	58.1	58.3	3.3	0.0	0.361058	68060	68737	779-784	1.000000	78.6
ycf4-cemA_p5	ycf4-cemA	5	ACAAAAAGCTGCGGAATTGG	CCGGCATTCCACCAATTAGT	20	20	45.0	50.0	57.8	58.2	0.0	0.0	0.390659	68060	68737	844-849	1.000000	78.3
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.0	56.2	0.0	0.0	1.831892	70622	71301	919-930	1.000000	67.2
petA-psbJ_p2	petA-psbJ	2	CATTACGTGTCCAAGGCCTT	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.2	56.2	13.8	0.0	1.972289	70622	71301	916-927	1.000000	66.1
petA-psbJ_p3	petA-psbJ	3	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATT	20	21	50.0	47.6	58.0	57.0	0.0	0.0	2.069983	70622	71301	919-930	1.000000	65.3
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATTC	20	22	50.0	50.0	58.0	58.1	0.0	0.0	2.122851	70622	71301	919-930	1.000000	64.9
petA-psbJ_p5	petA-psbJ	5	TTACGTGTCCAAGGCCTTTT	GCCGATACTACTGGAAGGAT	20	20	45.0	50.0	57.6	56.2	17.6	0.0	2.145211	70622	71301	914-925	1.000000	64.8
clpP_p1	clpP	1	TGGGCTGGTTTAGATTGATCC	TGTTACGTTTCCACATCAAAGT	21	22	47.6	36.4	57.7	56.5	0.0	0.0	4.798777	77491	79506	2183-2188	1.000000	43.3
clpP_p2	clpP	2	GGGCTGGTTTAGATTGATCCT	TGTTACGTTTCCACATCAAAGT	21	22	47.6	36.4	57.4	56.5	0.0	0.0	5.086375	77491	79506	2182-2187	1.000000	41.7
clpP_p3	clpP	3	TGGGCTGGTTTAGATTGATCC	TTGTTACGTTTCCACATCAAAGT	21	23	47.6	34.8	57.7	57.2	0.0	0.0	5.118651	77491	79506	2184-2189	1.000000	41.7
clpP_p4	clpP	4	GGGCTGGTTTAGATTGATCCT	TTGTTACGTTTCCACATCAAAGT	21	23	47.6	34.8	57.4	57.2	0.0	0.0	5.406249	77491	79506	2183-2188	1.000000	41.7
clpP_p5	clpP	5	TGGGCTGGTTTAGATTGATCC	TGTTACGTTTCCACATCAAAG	21	21	47.6	38.1	57.7	54.7	0.0	0.0	5.562786	77491	79506	2183-2188	1.000000	41.7
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGGATTCGAACCTACGACCA	CGGGCGCATCTCTTCTTATC	20	20	50.0	55.0	58.2	58.6	15.0	10.8	0.733237	115978	117544	1582-1714	1.000000	78.6
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	CCCCAAGTAGGATTCGAACC	CGGGCGCATCTCTTCTTATC	20	20	55.0	55.0	57.7	58.6	0.0	10.8	0.903805	115978	117544	1590-1722	1.000000	77.2
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	AGGATTCGAACCTACGACCA	GGGCGCATCTCTTCTTATCT	20	20	50.0	50.0	58.2	57.2	15.0	10.8	0.986373	115978	117544	1581-1713	1.000000	76.5
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	AGGATTCGAACCTACGACCA	ATCAAATATGTAGGGGGCGG	20	20	50.0	50.0	58.2	57.1	15.0	0.0	1.035734	115978	117544	1599-1731	1.000000	76.1
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CCCCAAGTAGGATTCGAACC	GGGCGCATCTCTTCTTATCT	20	20	55.0	50.0	57.7	57.2	0.0	10.8	1.156941	115978	117544	1589-1721	1.000000	75.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGAAGGGATATTGGACAGCA	TTAGAAGATGACATGCCGCC	20	20	50.0	50.0	57.0	58.0	0.0	0.0	1.076079	120711	121531	1009-1014	1.000000	72.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGAAGGGATATTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.0	57.9	0.0	0.0	1.153915	120711	121531	955-960	1.000000	72.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGAAGGGATATTGGACAGCA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	57.0	57.8	0.0	0.0	1.214642	120711	121531	948-953	1.000000	71.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGAAGGGATATTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	57.0	57.8	0.0	0.0	1.225933	120711	121531	954-959	1.000000	71.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGAAGGGATATTGGACAGCA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	57.0	57.8	0.0	0.0	1.225933	120711	121531	953-958	1.000000	71.5
ccsA-ndhD_p1	ccsA-ndhD	1	ATTCGGCAATTGTAGCGTCT	CCCGATTTCGTTCTGTCACT	20	20	45.0	50.0	58.0	57.9	7.4	0.0	0.104462	122663	122915	438-438	1.000000	80.8
ccsA-ndhD_p2	ccsA-ndhD	2	ATTCGGCAATTGTAGCGTCT	TGTACCCCGATTTCGTTCTG	20	20	45.0	50.0	58.0	57.6	7.4	0.0	0.397716	122663	122915	443-443	1.000000	78.4
ccsA-ndhD_p3	ccsA-ndhD	3	ATTCGGCAATTGTAGCGTCT	GTACCCCGATTTCGTTCTGT	20	20	45.0	50.0	58.0	57.6	7.4	0.0	0.399349	122663	122915	442-442	1.000000	78.4
ccsA-ndhD_p4	ccsA-ndhD	4	ATTCGGCAATTGTAGCGTCT	TACCCCGATTTCGTTCTGTC	20	20	45.0	50.0	58.0	57.3	7.4	0.0	0.684364	122663	122915	441-441	1.000000	76.2
ccsA-ndhD_p5	ccsA-ndhD	5	TTCGGCAATTGTAGCGTCTA	CCCGATTTCGTTCTGTCACT	20	20	45.0	50.0	57.3	57.9	7.4	0.0	0.738659	122663	122915	437-437	1.000000	75.7
ndhG-ndhI_p1	ndhG-ndhI	1	CCCTATCATTCAATATCTGGAA	GCACAATTTCGAATTCGCCT	22	20	36.4	45.0	52.8	57.7	0.0	22.9	7.463485	126199	126369	274-276	1.000000	44.7
ndhG-ndhI_p2	ndhG-ndhI	2	CCCTATCATTCAATATCTGGAAA	GCACAATTTCGAATTCGCCT	23	20	34.8	45.0	53.7	57.7	0.0	22.9	7.605766	126199	126369	274-276	1.000000	44.7
ndhG-ndhI_p3	ndhG-ndhI	3	CCCTATCATTCAATATCTGGAA	TCGCACAATTTCGAATTCGC	22	20	36.4	45.0	52.8	58.1	0.0	22.9	7.323811	126199	126369	276-278	1.000000	44.6
ndhG-ndhI_p4	ndhG-ndhI	4	CCCTATCATTCAATATCTGGAAA	TCGCACAATTTCGAATTCGC	23	20	34.8	45.0	53.7	58.1	0.0	22.9	7.466093	126199	126369	276-278	1.000000	44.6
ndhG-ndhI_p5	ndhG-ndhI	5	CCCTATCATTCAATATCTGGAA	TCAAATTGCTTTGGGTCGGT	22	20	36.4	45.0	52.8	58.3	0.0	0.0	7.502407	126199	126369	327-329	1.000000	43.9
ycf1_p1	ycf1	1	GTTTCCAGTCCCCAAACTGT	TGAGCGGAAAACAGATGCAT	20	20	50.0	45.0	57.9	58.2	3.6	0.0	0.242275	134859	134978	1092-1095	1.000000	84.6
ycf1_p2	ycf1	2	TTTGTGCCAAGGTTTCAGGT	TGAGCGGAAAACAGATGCAT	20	20	45.0	45.0	58.1	58.2	0.0	0.0	0.315608	134859	134978	1003-1006	1.000000	84.5
ycf1_p3	ycf1	3	CATCCCCTCATTAGTTGCCC	TGAGCGGAAAACAGATGCAT	20	20	55.0	45.0	58.0	58.2	0.0	0.0	0.188027	134859	134978	747-747	0.500000	58.5
ycf1_p4	ycf1	4	GTTGCCCATGTGACAGGTTA	TGAGCGGAAAACAGATGCAT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.270623	134859	134978	734-734	0.500000	57.8
ycf1_p5	ycf1	5	CATCCCCTCATTAGTTGCCC	ACACATGCCAAAGTGATGGA	20	20	55.0	45.0	58.0	57.7	0.0	7.5	0.304327	134859	134978	1192-1192	0.500000	57.6