Hotspot

trnS-UGA

LSC · 37105-37194 · 90 bp

Back to Flueggea

Pi 0.0556

MarkerSeek score 70.20

Primer available yes

Label trnS-UGA

Feature Metadata

label_name: trnS-UGA
feature_type: tRNA
region: LSC
length_bp: 90
variable_sites: 5
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA_p1	AGCCTCTAAGGGGTCCAAAT	GGGGATTGTACCACGAATCC	50.0	55.0	58.0	58.0	860-957 bp	1.0	81.5
trnS-UGA_p2	AGCCTCTAAGGGGTCCAAAT	GGGGGATTGTACCACGAATC	50.0	55.0	58.0	58.0	861-958 bp	1.0	81.5
trnS-UGA_p3	AGCCTCTAAGGGGTCCAAAT	CCATGTCCTATTCGGCGAAA	50.0	50.0	58.0	58.1	681-710 bp	1.0	81.1
trnS-UGA_p4	AGCCTCTAAGGGGTCCAAAT	CAACTCCTATGGCCATGTCC	50.0	55.0	58.0	58.0	693 bp	0.5	59.7
trnS-UGA_p5	ACCTACTGGACCAGAAGCTT	CAACTCCTATGGCCATGTCC	50.0	55.0	58.0	58.0	884 bp	0.5	59.5