Hotspot

rps16

LSC · 5501-6634 · 1134 bp

Back to Flueggea

Pi 0.33

MarkerSeek score 58.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1134
variable_sites: 32
pi: 0.33
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.09
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAGTGGACTCAGAAAGCCTG	AGTCGATCTGGTGGATAGGG	50.0	55.0	57.4	58.0	2091 bp	0.5	55.5
rps16_p2	AAGTGGACTCAGAAAGCCTG	GTCGATCTGGTGGATAGGGA	50.0	55.0	57.4	58.0	2090 bp	0.5	55.5
rps16_p3	AAGTGGACTCAGAAAGCCTG	AGATTGGCTTGGCAAATCCT	50.0	45.0	57.4	57.8	1754 bp	0.5	53.6
rps16_p4	AAGTGGACTCAGAAAGCCTG	TGGCTTGGCAAATCCTTTGA	50.0	45.0	57.4	58.3	1750 bp	0.5	53.3
rps16_p5	AAGTGGACTCAGAAAGCCTG	TGGTAGATTGGCTTGGCAAA	50.0	45.0	57.4	57.7	1758 bp	0.5	53.1