Hotspot

psaJ-rpl33

LSC · 69303-69753 · 451 bp

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Pi 0.0431

MarkerSeek score 77.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 19
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CATATCTATCCGTGGCACCG	GGACATCTTTACCCTTGGCC	55.0	55.0	58.0	58.2	592-645 bp	1.0	81.8
psaJ-rpl33_p2	TGGCACCGGTACTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	580-633 bp	1.0	80.2
psaJ-rpl33_p3	CATATCTATCCGTGGCACCG	GACATCTTTACCCTTGGCCA	55.0	50.0	58.0	57.5	591-644 bp	1.0	79.7
psaJ-rpl33_p4	GTGGCACCGGTACTAAGTAC	GGACATCTTTACCCTTGGCC	55.0	55.0	57.7	58.2	581-634 bp	1.0	79.7
psaJ-rpl33_p5	CATATCTATCCGTGGCACCG	CGGACATCTTTACCCTTGGC	55.0	55.0	58.0	58.6	593-646 bp	1.0	78.8