Hotspot

trnP-UGG-psaJ

LSC · 68715-69167 · 453 bp

Back to Flueggea

Pi 0.0214

MarkerSeek score 67.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 9
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	513-544 bp	1.0	84.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	512-543 bp	1.0	84.5
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	524-555 bp	1.0	84.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	523-554 bp	1.0	84.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	526-557 bp	1.0	84.4