Hotspot

psbE-petL

LSC · 67074-67859 · 786 bp

Back to Flueggea

Pi 0.133

MarkerSeek score 95.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 102
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 95.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.8	56.4	902-1048 bp	1.0	75.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGGTCAGACC	55.0	50.0	58.2	56.4	897-1043 bp	1.0	74.9
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	855-1001 bp	1.0	74.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	850-996 bp	1.0	74.2
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	AGTCGTATCTTGGTCAGACC	50.0	50.0	58.4	56.4	895-1041 bp	1.0	73.7