Hotspot

petA-psbJ

LSC · 65147-66276 · 1130 bp

Back to Flueggea

Pi 0.0324

MarkerSeek score 76.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 29
pi: 0.0324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATTACGTGTCCAAGGCCTT	ACTGGTATTCCTGTGATCGG	50.0	50.0	58.2	56.7	1150-1321 bp	1.0	74.2
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.2	56.7	1149-1320 bp	1.0	74.2
petA-psbJ_p3	GACCCATTACGTGTCCAAGG	ACTGGTATTCCTGTGATCGG	55.0	50.0	58.3	56.7	1154-1325 bp	1.0	73.5
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	CTGGTATTCCTGTGATCGGT	55.0	50.0	58.3	56.7	1153-1324 bp	1.0	73.5
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	CTGGTATTCCTGTGATCGGT	45.0	50.0	57.6	56.7	1147-1318 bp	1.0	72.8