Hotspot

trnM-CAU-atpE

LSC · 53986-54231 · 246 bp

Back to Flueggea

Pi 0.0943

MarkerSeek score 80.00

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 23
pi: 0.0943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	AGCTAGAGCACGAGTAGAGG	50.0	55.0	58.1	58.0	321-345 bp	1.0	85.0
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	CTAGAGCACGAGTAGAGGCT	50.0	55.0	58.1	58.0	319-343 bp	1.0	85.0
trnM-CAU-atpE_p3	GGCAGGAGTCATTGGTTCAA	AACTTGAGGAAAGCGGAAGG	50.0	50.0	58.1	58.1	380-404 bp	1.0	83.6
trnM-CAU-atpE_p4	TTGCTTTCATACGGCAGGAG	AGCTAGAGCACGAGTAGAGG	50.0	55.0	58.3	58.0	333-357 bp	1.0	83.4
trnM-CAU-atpE_p5	TTGCTTTCATACGGCAGGAG	CTAGAGCACGAGTAGAGGCT	50.0	55.0	58.3	58.0	331-355 bp	1.0	83.4