Hotspot

ndhC-trnV-UAC

LSC · 51919-53076 · 1158 bp

Back to Flueggea

Pi 0.0714
MarkerSeek score 80.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1158
variable_sites
70
pi
0.0714
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATATTCGT GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.1 58.7 1075-1215 bp 1.0 48.9
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.1 57.8 1087-1227 bp 1.0 48.8
ndhC-trnV-UAC_p3 TGCCCAGAAAATATCATATTCGT GAAGGTCTACGGTTCGAGTC 34.8 55.0 56.1 57.5 1086-1226 bp 1.0 48.8
ndhC-trnV-UAC_p4 ATGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.6 57.8 1088-1228 bp 1.0 48.8
ndhC-trnV-UAC_p5 TGCCCAGAAAATATCATATTCGT AAGGTCTACGGTTCGAGTCC 34.8 55.0 56.1 58.8 1085-1225 bp 1.0 48.8