primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	156	495	313-406	1.000000	90.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	156	495	327-420	1.000000	90.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	156	495	326-419	1.000000	90.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	156	495	328-421	1.000000	90.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	156	495	316-409	1.000000	90.4
rps16_p1	rps16	1	AAGTGGACTCAGAAAGCCTG	AGTCGATCTGGTGGATAGGG	20	20	50.0	55.0	57.4	58.0	0.0	0.0	0.560425	5501	6634	2091-2091	0.500000	55.5
rps16_p2	rps16	2	AAGTGGACTCAGAAAGCCTG	GTCGATCTGGTGGATAGGGA	20	20	50.0	55.0	57.4	58.0	0.0	0.0	0.561121	5501	6634	2090-2090	0.500000	55.5
rps16_p3	rps16	3	AAGTGGACTCAGAAAGCCTG	AGATTGGCTTGGCAAATCCT	20	20	50.0	45.0	57.4	57.8	0.0	13.3	0.800802	5501	6634	1754-1754	0.500000	53.6
rps16_p4	rps16	4	AAGTGGACTCAGAAAGCCTG	TGGCTTGGCAAATCCTTTGA	20	20	50.0	45.0	57.4	58.3	0.0	16.2	0.843379	5501	6634	1750-1750	0.500000	53.3
rps16_p5	rps16	5	AAGTGGACTCAGAAAGCCTG	TGGTAGATTGGCTTGGCAAA	20	20	50.0	45.0	57.4	57.7	0.0	13.3	0.858875	5501	6634	1758-1758	0.500000	53.1
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	TGAACCGAGAGATCCCTTAAC	20	21	50.0	47.6	58.0	56.8	0.0	0.0	2.170357	9317	10091	800-881	1.000000	74.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	TGAACCGAGAGATCCCTTAAC	20	21	50.0	47.6	58.0	56.8	0.0	0.0	2.171745	9317	10091	799-880	1.000000	74.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	TGAACCGAGAGATCCCTTAACT	20	22	50.0	45.5	58.0	58.3	0.0	0.0	2.294996	9317	10091	800-881	1.000000	73.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	TGAACCGAGAGATCCCTTAACT	20	22	50.0	45.5	58.0	58.3	0.0	0.0	2.296384	9317	10091	799-880	1.000000	73.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGAACCGGAAAGAGAGGGAT	TGAACCGAGAGATCCCTTAAC	20	21	50.0	47.6	57.7	56.8	9.5	0.0	2.421545	9317	10091	858-939	1.000000	72.6
trnS-UGA_p1	trnS-UGA	1	AGCCTCTAAGGGGTCCAAAT	GGGGATTGTACCACGAATCC	20	20	50.0	55.0	58.0	58.0	0.0	12.3	0.049748	37105	37194	860-957	1.000000	81.5
trnS-UGA_p2	trnS-UGA	2	AGCCTCTAAGGGGTCCAAAT	GGGGGATTGTACCACGAATC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.049748	37105	37194	861-958	1.000000	81.5
trnS-UGA_p3	trnS-UGA	3	AGCCTCTAAGGGGTCCAAAT	CCATGTCCTATTCGGCGAAA	20	20	50.0	50.0	58.0	58.1	0.0	27.4	0.064816	37105	37194	681-710	1.000000	81.1
trnS-UGA_p4	trnS-UGA	4	AGCCTCTAAGGGGTCCAAAT	CAACTCCTATGGCCATGTCC	20	20	50.0	55.0	58.0	58.0	0.0	29.0	0.040048	37105	37194	693-693	0.500000	59.7
trnS-UGA_p5	trnS-UGA	5	ACCTACTGGACCAGAAGCTT	CAACTCCTATGGCCATGTCC	20	20	50.0	55.0	58.0	58.0	28.6	29.0	0.058623	37105	37194	884-884	0.500000	59.5
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	0.0	1.1	1.036941	37701	38445	704-896	1.000000	78.7
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	56.8	57.8	0.0	1.1	1.328499	37701	38445	707-899	1.000000	76.4
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	56.8	57.8	0.0	1.1	1.328499	37701	38445	705-897	1.000000	76.4
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	58.4	57.8	0.0	1.1	1.549901	37701	38445	708-900	1.000000	74.6
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	57.6	57.8	0.0	1.1	1.593372	37701	38445	706-898	1.000000	74.2
rps14-psaB_p1	rps14-psaB	1	AATGGTAAAGGGGCTCCTCT	AATTTCCTGGCGCGGATATT	20	20	50.0	45.0	58.0	58.0	5.0	8.8	0.031664	39255	39371	706-714	1.000000	83.4
rps14-psaB_p2	rps14-psaB	2	TCCTACCCTCTCCCTCTTCT	AATTTCCTGGCGCGGATATT	20	20	55.0	45.0	58.0	58.0	0.0	8.8	0.021991	39255	39371	995-995	0.500000	59.8
rps14-psaB_p3	rps14-psaB	3	TCCTACCCTCTCCCTCTTCT	CCCGGCTGGTTAAATGCTAT	20	20	55.0	50.0	58.0	58.0	0.0	11.6	0.021991	39255	39371	1504-1504	0.500000	59.8
rps14-psaB_p4	rps14-psaB	4	TCTCCTACCCTCTCCCTCTT	AATTTCCTGGCGCGGATATT	20	20	55.0	45.0	58.0	58.0	0.0	8.8	0.021991	39255	39371	997-997	0.500000	59.8
rps14-psaB_p5	rps14-psaB	5	TCTCCTACCCTCTCCCTCTT	CCCGGCTGGTTAAATGCTAT	20	20	55.0	50.0	58.0	58.0	0.0	11.6	0.021991	39255	39371	1506-1506	0.500000	59.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCCAGAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	23	20	34.8	55.0	56.1	58.7	0.0	0.0	5.564602	51919	53076	1075-1215	1.000000	48.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	56.1	57.8	0.0	0.0	5.078536	51919	53076	1087-1227	1.000000	48.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCCAGAAAATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	23	20	34.8	55.0	56.1	57.5	0.0	0.0	5.422308	51919	53076	1086-1226	1.000000	48.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ATGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	56.6	57.8	0.0	0.0	5.595649	51919	53076	1088-1228	1.000000	48.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCCAGAAAATATCATATTCGT	AAGGTCTACGGTTCGAGTCC	23	20	34.8	55.0	56.1	58.8	0.0	0.0	5.709077	51919	53076	1085-1225	1.000000	48.8
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	GGCAGGAGTCATTGGTTCAA	AGCTAGAGCACGAGTAGAGG	20	20	50.0	55.0	58.1	58.0	0.0	4.0	0.128507	53986	54231	321-345	1.000000	85.0
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	GGCAGGAGTCATTGGTTCAA	CTAGAGCACGAGTAGAGGCT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.128507	53986	54231	319-343	1.000000	85.0
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	GGCAGGAGTCATTGGTTCAA	AACTTGAGGAAAGCGGAAGG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.192740	53986	54231	380-404	1.000000	83.6
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TTGCTTTCATACGGCAGGAG	AGCTAGAGCACGAGTAGAGG	20	20	50.0	55.0	58.3	58.0	0.0	4.0	0.300368	53986	54231	333-357	1.000000	83.4
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TTGCTTTCATACGGCAGGAG	CTAGAGCACGAGTAGAGGCT	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.300368	53986	54231	331-355	1.000000	83.4
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	AGATCCACCAGGGCAAAAAG	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.166845	62366	63247	491-1000	1.000000	83.6
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	62366	63247	492-1001	1.000000	83.6
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	GATCCACCAGGGCAAAAAGA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	62366	63247	490-999	1.000000	83.6
ycf4-cemA_p4	ycf4-cemA	4	TTGGCCTATTTCTTGCGTGT	AAAAAGAGATCCACCAGGGC	20	20	45.0	50.0	58.1	57.8	3.3	0.0	0.317940	62366	63247	497-1006	1.000000	82.4
ycf4-cemA_p5	ycf4-cemA	5	TTGGCCTATTTCTTGCGTGT	CCACCAGGGCAAAAAGACTA	20	20	45.0	50.0	58.1	57.7	3.3	0.0	0.382078	62366	63247	487-996	1.000000	81.9
petA-psbJ_p1	petA-psbJ	1	CATTACGTGTCCAAGGCCTT	ACTGGTATTCCTGTGATCGG	20	20	50.0	50.0	58.2	56.7	13.8	0.0	1.458444	65147	66276	1150-1321	1.000000	74.2
petA-psbJ_p2	petA-psbJ	2	CATTACGTGTCCAAGGCCTT	CTGGTATTCCTGTGATCGGT	20	20	50.0	50.0	58.2	56.7	13.8	0.0	1.458444	65147	66276	1149-1320	1.000000	74.2
petA-psbJ_p3	petA-psbJ	3	GACCCATTACGTGTCCAAGG	ACTGGTATTCCTGTGATCGG	20	20	55.0	50.0	58.3	56.7	0.0	0.0	1.545498	65147	66276	1154-1325	1.000000	73.5
petA-psbJ_p4	petA-psbJ	4	GACCCATTACGTGTCCAAGG	CTGGTATTCCTGTGATCGGT	20	20	55.0	50.0	58.3	56.7	0.0	0.0	1.545498	65147	66276	1153-1324	1.000000	73.5
petA-psbJ_p5	petA-psbJ	5	TTACGTGTCCAAGGCCTTTT	CTGGTATTCCTGTGATCGGT	20	20	45.0	50.0	57.6	56.7	17.6	0.0	1.631366	65147	66276	1147-1318	1.000000	72.8
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGGTCAGACC	20	20	50.0	50.0	57.8	56.4	24.9	13.3	1.774913	67074	67859	902-1048	1.000000	75.2
psbE-petL_p2	psbE-petL	2	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGGTCAGACC	20	20	55.0	50.0	58.2	56.4	0.9	13.3	1.816501	67074	67859	897-1043	1.000000	74.9
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	67074	67859	855-1001	1.000000	74.5
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	20	20	55.0	45.0	58.2	56.3	0.9	12.5	1.909925	67074	67859	850-996	1.000000	74.2
psbE-petL_p5	psbE-petL	5	TCTCCTGTGCTTCCAGACAT	AGTCGTATCTTGGTCAGACC	20	20	50.0	50.0	58.4	56.4	0.9	13.3	1.969249	67074	67859	895-1041	1.000000	73.7
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	CGGTGCCACGGATAGATATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.103463	68715	69167	513-544	1.000000	84.5
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	CGGTGCCACGGATAGATATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.104807	68715	69167	512-543	1.000000	84.5
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	CGGTGCCACGGATAGATATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.111499	68715	69167	524-555	1.000000	84.4
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	CGGTGCCACGGATAGATATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.112832	68715	69167	523-554	1.000000	84.4
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	CGGTGCCACGGATAGATATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.112832	68715	69167	526-557	1.000000	84.4
psaJ-rpl33_p1	psaJ-rpl33	1	CATATCTATCCGTGGCACCG	GGACATCTTTACCCTTGGCC	20	20	55.0	55.0	58.0	58.2	12.9	0.0	0.239740	69303	69753	592-645	1.000000	81.8
psaJ-rpl33_p2	psaJ-rpl33	2	TGGCACCGGTACTAAGTACT	GGACATCTTTACCCTTGGCC	20	20	50.0	55.0	57.8	58.2	6.9	0.0	0.456685	69303	69753	580-633	1.000000	80.2
psaJ-rpl33_p3	psaJ-rpl33	3	CATATCTATCCGTGGCACCG	GACATCTTTACCCTTGGCCA	20	20	55.0	50.0	58.0	57.5	12.9	0.0	0.511115	69303	69753	591-644	1.000000	79.7
psaJ-rpl33_p4	psaJ-rpl33	4	GTGGCACCGGTACTAAGTAC	GGACATCTTTACCCTTGGCC	20	20	55.0	55.0	57.7	58.2	6.9	0.0	0.520295	69303	69753	581-634	1.000000	79.7
psaJ-rpl33_p5	psaJ-rpl33	5	CATATCTATCCGTGGCACCG	CGGACATCTTTACCCTTGGC	20	20	55.0	55.0	58.0	58.6	12.9	0.0	0.624182	69303	69753	593-646	1.000000	78.8
rpl36-rps8_p1	rpl36-rps8	1	ATTCGCCCTCGTCTACGTAT	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	58.4	56.8	0.0	6.3	1.573346	82001	82493	615-625	1.000000	71.0
rpl36-rps8_p2	rpl36-rps8	2	AATTCGCCCTCGTCTACGTA	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	58.6	56.8	2.0	6.3	1.793134	82001	82493	616-626	1.000000	69.3
rpl36-rps8_p3	rpl36-rps8	3	TAATTCGCCCTCGTCTACGT	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	58.6	56.8	2.0	6.3	1.793134	82001	82493	617-627	1.000000	69.3
rpl36-rps8_p4	rpl36-rps8	4	GCCCTCGTCTACGTATCAGT	AATGACAGATCGAGAGGCTC	20	20	55.0	50.0	58.7	56.8	0.0	6.3	1.868559	82001	82493	611-621	1.000000	68.7
rpl36-rps8_p5	rpl36-rps8	5	ATAATTCGCCCTCGTCTACG	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	57.3	56.8	0.0	6.3	1.876730	82001	82493	618-628	1.000000	68.6
rpl14-rpl16_p1	rpl14-rpl16	1	TCTGCTACATTTAAATAGGTTTGAGA	TCACTAGCAGCGTCCAAAAT	26	20	30.8	45.0	56.7	57.5	0.0	0.0	7.790380	83462	83592	216-220	1.000000	44.8
rpl14-rpl16_p2	rpl14-rpl16	2	CTGCTACATTTAAATAGGTTTGAGA	TTCACTAGCAGCGTCCAAAA	25	20	32.0	45.0	55.4	57.8	0.0	0.0	7.843984	83462	83592	216-220	1.000000	44.8
rpl14-rpl16_p3	rpl14-rpl16	3	TCTGCTACATTTAAATAGGTTTGAGA	TTCACTAGCAGCGTCCAAAA	26	20	30.8	45.0	56.7	57.8	0.0	0.0	7.565965	83462	83592	217-221	1.000000	44.7
rpl14-rpl16_p4	rpl14-rpl16	4	TCTGCTACATTTAAATAGGTTTGAGA	TTTCACTAGCAGCGTCCAAA	26	20	30.8	45.0	56.7	57.8	0.0	0.0	7.565965	83462	83592	218-222	1.000000	44.7
rpl14-rpl16_p5	rpl14-rpl16	5	CTGCTACATTTAAATAGGTTTGAGA	TTTCACTAGCAGCGTCCAAA	25	20	32.0	45.0	55.4	57.8	0.0	0.0	7.843984	83462	83592	217-221	1.000000	44.7
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	110869	111515	788-831	1.000000	79.2
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	110869	111515	825-868	1.000000	78.6
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	20	20	55.0	50.0	57.8	58.1	18.9	0.0	0.311620	110869	111515	829-872	1.000000	78.4
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	110869	111515	739-782	1.000000	78.1
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	20	20	55.0	50.0	57.7	58.1	18.7	0.0	0.381540	110869	111515	866-909	1.000000	77.8
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	132583	133229	788-831	1.000000	79.2
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	132583	133229	825-868	1.000000	78.6
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.1	57.8	0.0	18.9	0.311620	132583	133229	829-872	1.000000	78.4
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	132583	133229	739-782	1.000000	78.1
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	20	20	50.0	55.0	58.1	57.7	0.0	18.7	0.381540	132583	133229	866-909	1.000000	77.8