Hotspot

ycf1

SSC · 122298-127649 · 5352 bp

Back to Flemingia

Pi 0.00571

MarkerSeek score 42.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5352
variable_sites: 56
pi: 0.00571
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTTTCCATCACTTTGACATGT	ACCGATTCAACAACAAAGATCT	34.8	36.4	56.6	56.3	232-241 bp	1.0	44.7
ycf1_p2	TCTTTTCCATCACTTTGACATGT	AACCGATTCAACAACAAAGATCT	34.8	34.8	56.6	57.0	233-242 bp	1.0	44.7
ycf1_p3	TCTTTTCCATCACTTTGACATGT	ACCGATTCAACAACAAAGATCTT	34.8	34.8	56.6	57.0	232-241 bp	1.0	44.7
ycf1_p4	TTCTTTTCCATCACTTTGACATGT	ACCGATTCAACAACAAAGATCT	33.3	36.4	57.3	56.3	233-242 bp	1.0	44.7
ycf1_p5	CTTTTCCATCACTTTGACATGT	ACCGATTCAACAACAAAGATCT	36.4	36.4	55.2	56.3	231-240 bp	1.0	44.7