Hotspot

rps15-ycf1

SSC · 121912-122297 · 386 bp

Back to Flemingia

Pi 0.0299

MarkerSeek score 69.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 386
variable_sites: 19
pi: 0.0299
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGATCCCGGGTTTTCTTCTT	ACCCATAATGGAAGCCGTTT	50.0	45.0	57.9	57.5	514-515 bp	1.0	78.3
rps15-ycf1_p2	CGATCCCGGGTTTTCTTCTT	CCCATAATGGAAGCCGTTTC	50.0	50.0	57.9	56.8	513-514 bp	1.0	73.3
rps15-ycf1_p3	CGATCCCGGGTTTTCTTCTT	TACCCATAATGGAAGCCGTT	50.0	45.0	57.9	56.6	515-516 bp	1.0	71.3
rps15-ycf1_p4	CGATCCCGGGTTTTCTTCTT	ACCCATAATGGAAGCCGTTTC	50.0	47.6	57.9	58.6	514-515 bp	1.0	69.5
rps15-ycf1_p5	CGATCCCGGGTTTTCTTCTT	CCCATAATGGAAGCCGTTTCA	50.0	47.6	57.9	58.6	513-514 bp	1.0	69.5