Hotspot

rpl20-rps12

LSC · 67941-68753 · 813 bp

Back to Flemingia

Pi 0.00697
MarkerSeek score 55.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
813
variable_sites
10
pi
0.00697
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTTTTCTCCGTTTCCGAGCT ACAAAATCCCCAGCTCTTCG 45.0 50.0 57.8 58.2 910-922 bp 1.0 78.4
rpl20-rps12_p2 TTTTTCTCCGTTTCCGAGCT AAGACAAGACAGCCAATCCG 45.0 50.0 57.8 58.2 937-949 bp 1.0 78.2
rpl20-rps12_p3 TTTTTCTCCGTTTCCGAGCT GACAAGACAGCCAATCCGAA 45.0 50.0 57.8 58.2 935-947 bp 1.0 78.2
rpl20-rps12_p4 AAGTCTTGAATGAGCTCCGC ACAAAATCCCCAGCTCTTCG 50.0 50.0 58.3 58.2 957-969 bp 1.0 78.1
rpl20-rps12_p5 AAGTCTTGAATGAGCTCCGC AAGACAAGACAGCCAATCCG 50.0 50.0 58.3 58.2 984-996 bp 1.0 78.0