Hotspot

rpl33-rps18

LSC · 66758-67005 · 248 bp

Back to Flemingia

Pi 0.0565

MarkerSeek score 80.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 23
pi: 0.0565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCTGTCCCCCGATTG	55.0	50.0	58.5	57.6	421-447 bp	1.0	77.8
rpl33-rps18_p2	CGACACAATACGCCTAGTCG	ATTCTGTCCCCCGATTGGAT	55.0	50.0	58.5	58.5	418-444 bp	1.0	77.4
rpl33-rps18_p3	CGACACAATACGCCTAGTCG	CAATTCTGTCCCCCGATTGG	55.0	55.0	58.5	58.6	420-446 bp	1.0	76.3
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	AATTCTGTCCCCCGATTGGA	55.0	50.0	58.5	58.7	419-445 bp	1.0	75.5
rpl33-rps18_p5	GACACAATACGCCTAGTCGA	TCAATTCTGTCCCCCGATTG	50.0	50.0	57.2	57.6	420-446 bp	1.0	75.4