Hotspot

psaJ-rpl33

LSC · 66049-66556 · 508 bp

Back to Flemingia

Pi 0.00492

MarkerSeek score 51.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 5
pi: 0.00492
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	CACAACCGGTACACTCCAAA	50.0	50.0	58.0	58.0	678-683 bp	1.0	80.5
psaJ-rpl33_p2	TTGGCGGGTCTCTTGATAGA	CACAACCGGTACACTCCAAA	50.0	50.0	58.1	58.0	630-635 bp	1.0	79.8
psaJ-rpl33_p3	TACTCTATGGTTCGGGGCTT	CACAACCGGTACACTCCAAA	50.0	50.0	57.8	58.0	649-654 bp	1.0	79.5
psaJ-rpl33_p4	CTCTATGGTTCGGGGCTTTG	CACAACCGGTACACTCCAAA	55.0	50.0	58.3	58.0	647-652 bp	1.0	78.2
psaJ-rpl33_p5	ATATCTTTCCGTGGCACCTG	CACAACCGGTACACTCCAAA	50.0	50.0	57.7	58.0	676-681 bp	1.0	78.2