Hotspot

trnQ-UUG-rps16

LSC · 55297-55818 · 522 bp

Back to Flemingia

Pi 0.0134

MarkerSeek score 61.40

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 11
pi: 0.0134
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	590-602 bp	1.0	83.0
trnQ-UUG-rps16_p2	GTCAAATCGAAAATGGGGCG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.1	58.0	651-663 bp	1.0	82.5
trnQ-UUG-rps16_p3	GAGGTTCGAATCCTTCCGTC	AAGTCTTTCAAGTCGCACGT	55.0	45.0	58.1	58.1	592-604 bp	1.0	82.5
trnQ-UUG-rps16_p4	GAGGTTCGAATCCTTCCGTC	AGTCTTTCAAGTCGCACGTT	55.0	45.0	58.1	58.1	591-603 bp	1.0	82.5
trnQ-UUG-rps16_p5	GTCAAATCGAAAATGGGGCG	AGTCTTTCAAGTCGCACGTT	50.0	45.0	58.1	58.1	652-664 bp	1.0	82.0