Hotspot

trnS-UGA-psbC

LSC · 26342-26544 · 203 bp

Back to Flemingia

Pi 0.00575
MarkerSeek score 52.70
Primer available yes
Label trnS-UGA-psbC

Feature Metadata

label_name
trnS-UGA-psbC
feature_type
igs
region
LSC
length_bp
203
variable_sites
2
pi
0.00575
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbC_p1 GAGAGATGGCTGAGTGGTTG ACCTGTTCTTTCCATGACTCC 55.0 47.6 58.0 57.6 316-326 bp 1.0 71.4
trnS-UGA-psbC_p2 ATAGCCCCGGTCTTGAAAAC ACCTGTTCTTTCCATGACTCC 50.0 47.6 57.9 57.6 296-306 bp 1.0 70.8
trnS-UGA-psbC_p3 GCTTTTGGAGAGATGGCTGA ACCTGTTCTTTCCATGACTCC 50.0 47.6 57.9 57.6 323-333 bp 1.0 70.5
trnS-UGA-psbC_p4 AGCTTTTGGAGAGATGGCTG ACCTGTTCTTTCCATGACTCC 50.0 47.6 57.9 57.6 324-334 bp 1.0 70.5
trnS-UGA-psbC_p5 GAGAGATGGCTGAGTGGTTG ATTTATGGCACGCGGGAAG 55.0 52.6 58.0 58.6 386-396 bp 1.0 69.5