Hotspot

ycf3-psaA

LSC · 18854-19650 · 797 bp

Back to Flemingia

Pi 0.00523

MarkerSeek score 63.10

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 8
pi: 0.00523
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	AATGACCAGGTTTTGCCCAT	50.0	45.0	58.0	58.0	958-995 bp	1.0	80.8
ycf3-psaA_p2	AGCCCTTACCCCTGGTAATT	ATGACCAGGTTTTGCCCATT	50.0	45.0	58.0	58.0	957-994 bp	1.0	80.8
ycf3-psaA_p3	AGCTATAGCCCTTACCCCTG	AATGACCAGGTTTTGCCCAT	55.0	45.0	58.0	58.0	964-1001 bp	1.0	80.6
ycf3-psaA_p4	AGCTATAGCCCTTACCCCTG	ATGACCAGGTTTTGCCCATT	55.0	45.0	58.0	58.0	963-1000 bp	1.0	80.6
ycf3-psaA_p5	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	945-982 bp	1.0	80.1