Hotspot

atpE-trnM-CAU

LSC · 9489-9625 · 137 bp

Back to Flemingia

Pi 0.00852

MarkerSeek score 53.40

Primer available yes

Label atpE-trnM-CAU

Feature Metadata

label_name: atpE-trnM-CAU
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 2
pi: 0.00852
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-trnM-CAU_p1	CGAGCTAGGACACGAGTAGA	TGGAGGCGGTGATAAATGAC	55.0	50.0	58.1	57.7	262-270 bp	1.0	79.9
atpE-trnM-CAU_p2	TAGACGAGCTAGGACACGAG	TGGAGGCGGTGATAAATGAC	55.0	50.0	58.1	57.7	266-274 bp	1.0	79.9
atpE-trnM-CAU_p3	CGAGCTAGGACACGAGTAGA	TATTGCTTTCATACGGCGGG	55.0	50.0	58.1	58.4	220-228 bp	1.0	79.8
atpE-trnM-CAU_p4	CGAGCTAGGACACGAGTAGA	GGAGGCGGTGATAAATGACC	55.0	55.0	58.1	58.4	261-269 bp	1.0	79.3
atpE-trnM-CAU_p5	TAGACGAGCTAGGACACGAG	GGAGGCGGTGATAAATGACC	55.0	55.0	58.1	58.4	265-273 bp	1.0	79.2