Hotspot

trnK-UUU-rbcL

LSC · 4408-5363 · 956 bp

Back to Flemingia

Pi 0.00981

MarkerSeek score 58.40

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 18
pi: 0.00981
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGACGAACCAATTGCTTCA	AGTCCTGAATTAGCTGCTGC	45.0	50.0	58.0	58.0	1115-1116 bp	1.0	82.3
trnK-UUU-rbcL_p2	TGGACGAACCAATTGCTTCA	GAGGCTAGCAAATGGAGTCC	45.0	55.0	58.0	58.0	1130-1131 bp	1.0	82.2
trnK-UUU-rbcL_p3	CCGCTCTTAAAAGCCGAGTA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.7	58.0	1073-1074 bp	1.0	80.3
trnK-UUU-rbcL_p4	CCGCTCTTAAAAGCCGAGTA	GAGGCTAGCAAATGGAGTCC	50.0	55.0	57.7	58.0	1088-1089 bp	1.0	80.2
trnK-UUU-rbcL_p5	TAGAGCCGCTCTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.7	58.0	1078-1079 bp	1.0	80.1