Hotspot

ycf1

IRb · 110413-112362 · 1950 bp

Back to Flacourtia

Pi 0.0213

MarkerSeek score 71.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1950
variable_sites: 56
pi: 0.0213
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTCGTTTCTAGTCTATCTGTTTCT	CAATGGAGTCGGCTTTACGA	36.0	50.0	57.5	58.0	2029-2095 bp	1.0	45.0
ycf1_p2	TGTCGTTTCTAGTCTATCTGT	CAATGGAGTCGGCTTTACGA	38.1	50.0	53.5	58.0	2029-2095 bp	1.0	45.0
ycf1_p3	TGTCGTTTCTAGTCTATCTGTTTCT	GGAGTCGGCTTTACGAGTTT	36.0	50.0	57.5	57.9	2025-2091 bp	1.0	45.0
ycf1_p4	TGTCGTTTCTAGTCTATCTGT	GGAGTCGGCTTTACGAGTTT	38.1	50.0	53.5	57.9	2025-2091 bp	1.0	45.0
ycf1_p5	TGTCGTTTCTAGTCTATCTGTTTCT	ACCAATGGAGTCGGCTTTAC	36.0	50.0	57.5	57.9	2031-2097 bp	1.0	45.0