Hotspot

trnN-GUU-trnR-ACG

IRa · 131031-131695 · 665 bp

Back to Flacourtia

Pi 0.00924

MarkerSeek score 69.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 665
variable_sites: 10
pi: 0.00924
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	55.0	50.0	58.0	58.1	814-842 bp	1.0	83.1
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	778-806 bp	1.0	82.1
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	765-793 bp	1.0	82.1
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.8	58.1	778-806 bp	1.0	82.0
trnN-GUU-trnR-ACG_p5	CTGGTCGTAGGTTCGAATCC	AACAGATCCAGTGGAGACGA	55.0	50.0	57.8	58.1	766-794 bp	1.0	81.7