Hotspot

ndhF-trnL-UAG

SSC · 114509-115179 · 671 bp

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Pi 0.0147
MarkerSeek score 57.70
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
671
variable_sites
18
pi
0.0147
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CTGGAAGTGTAACGAAAGAT AGCGTGTCTACCGATTTCAC 40.0 50.0 53.0 58.0 752-773 bp 1.0 41.9
ndhF-trnL-UAG_p2 CTGGAAGTGTAACGAAAGAT CAGCGTGTCTACCGATTTCA 40.0 50.0 53.0 58.0 753-774 bp 1.0 41.9
ndhF-trnL-UAG_p3 CTGGAAGTGTAACGAAAGAT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 53.0 57.9 762-783 bp 1.0 41.9
ndhF-trnL-UAG_p4 CTGGAAGTGTAACGAAAGAT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 53.0 57.8 761-782 bp 1.0 41.9
ndhF-trnL-UAG_p5 CTGGAAGTGTAACGAAAGAT CTAAGAGCAGCGTGTCTACC 40.0 55.0 53.0 57.8 760-781 bp 1.0 41.9