Hotspot

rpoA-rps11

LSC · 79364-79435 · 72 bp

Back to Flacourtia

Pi 0.0258

MarkerSeek score 63.40

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 3
pi: 0.0258
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	TTCCACTGTAGTGTCCGAGT	GGATGTAACCCCTATGCCAC	50.0	55.0	58.0	57.7	176-177 bp	1.0	79.6
rpoA-rps11_p2	ACTTCCACTGTAGTGTCCGA	GGATGTAACCCCTATGCCAC	50.0	55.0	58.0	57.7	178-179 bp	1.0	79.5
rpoA-rps11_p3	ACTTCCACTGTAGTGTCCGA	GTCCGGGATGTAACCCCTAT	50.0	55.0	58.0	58.3	183-184 bp	1.0	79.5
rpoA-rps11_p4	TTCCACTGTAGTGTCCGAGT	GTCCGGGATGTAACCCCTAT	50.0	55.0	58.0	58.3	181-182 bp	1.0	79.5
rpoA-rps11_p5	TTCCACTGTAGTGTCCGAGT	TCCGGGATGTAACCCCTATG	50.0	55.0	58.0	58.3	180-181 bp	1.0	79.5