Hotspot

petA-psbJ

LSC · 62973-63912 · 940 bp

Back to Flacourtia

Pi 0.0258

MarkerSeek score 79.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 40
pi: 0.0258
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	847-1246 bp	1.0	81.9
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	849-1248 bp	1.0	81.9
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	841-1240 bp	1.0	80.7
petA-psbJ_p4	CCATTACGTGTCCAAGGTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	846-1245 bp	1.0	77.9
petA-psbJ_p5	CCCATTACGTGTCCAAGGTC	ATGGCTCCTATTCCGGATTG	55.0	50.0	58.3	57.1	856-1255 bp	1.0	77.9