Hotspot

ndhC-trnV-UAC

LSC · 50295-51566 · 1272 bp

Back to Flacourtia

Pi 0.0113

MarkerSeek score 59.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1272
variable_sites: 20
pi: 0.0113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGCCCTTTTCTAATTGGGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1039-1440 bp	1.0	81.7
ndhC-trnV-UAC_p2	GGCCCTTTTCTAATTGGGGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1038-1439 bp	1.0	79.4
ndhC-trnV-UAC_p3	TGGCCCTTTTCTAATTGGGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.8	57.5	1038-1439 bp	1.0	79.0
ndhC-trnV-UAC_p4	TGGCCCTTTTCTAATTGGGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.8	58.7	1027-1428 bp	1.0	77.9
ndhC-trnV-UAC_p5	TGGCCCTTTTCTAATTGGGG	AAGGTCTACGGTTCGAGTCC	50.0	55.0	57.8	58.8	1037-1438 bp	1.0	76.7