Hotspot

petN-psbM

LSC · 27549-28870 · 1322 bp

Back to Flacourtia

Pi 0.0475

MarkerSeek score 80.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1322
variable_sites: 63
pi: 0.0475
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	907-1489 bp	1.0	75.7
petN-psbM_p2	CTTGGGCTGCTTTGATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	895-1477 bp	1.0	75.7
petN-psbM_p3	TTGGGCTGCTTTGATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	894-1476 bp	1.0	75.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	846-1429 bp	1.0	74.4
petN-psbM_p5	GGGGCCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	929-1511 bp	1.0	73.8