Hotspot

trnC-GCA-petN

LSC · 27225-27458 · 234 bp

Back to Flacourtia

Pi 0.0185

MarkerSeek score 63.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 8
pi: 0.0185
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	300-459 bp	1.0	83.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CTACCATCAAAGCAGCCCAA	50.0	50.0	58.1	58.2	313-472 bp	1.0	83.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TACCATCAAAGCAGCCCAAG	50.0	50.0	58.1	58.2	312-471 bp	1.0	83.7
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	361-520 bp	1.0	81.8
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGACTACCATCAAAGCAGCC	50.0	50.0	58.1	57.6	316-475 bp	1.0	81.7