Hotspot

trnS-GCU-trnR-UCU

LSC · 7178-8774 · 1597 bp

Back to Flacourtia

Pi 0.00952

MarkerSeek score 62.80

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1597
variable_sites: 25
pi: 0.00952
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGGCGCTTTA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	1647-1673 bp	1.0	66.8
trnS-GCU-trnR-UCU_p2	ATCAACGGAACCGGAAAGAG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.9	56.9	1706-1732 bp	1.0	66.2
trnS-GCU-trnR-UCU_p3	ACCAAGTCATCAACGGAACC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.1	56.9	1714-1740 bp	1.0	66.1
trnS-GCU-trnR-UCU_p4	ATTAGCAATCCGGCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	45.0	45.5	58.0	57.9	1653-1679 bp	1.0	66.1
trnS-GCU-trnR-UCU_p5	ATCAACGGAACCGGAAAGAG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	57.9	57.9	1712-1738 bp	1.0	65.5