Hotspot

trnK-UUU-trnQ-UUG

LSC · 4310-5814 · 1505 bp

Back to Flacourtia

Pi 0.0108
MarkerSeek score 60.50
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1505
variable_sites
26
pi
0.0108
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 TGTAAAAGCTGTTAGCCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1540-1587 bp 1.0 83.2
trnK-UUU-trnQ-UUG_p2 AAAGCTGTTAGCCGCACTTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1536-1583 bp 1.0 82.7
trnK-UUU-trnQ-UUG_p3 AAGCTGTTAGCCGCACTTAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1535-1582 bp 1.0 82.7
trnK-UUU-trnQ-UUG_p4 AGCTGTTAGCCGCACTTAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1534-1581 bp 1.0 82.7
trnK-UUU-trnQ-UUG_p5 TAAAAGCTGTTAGCCGCACT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1538-1585 bp 1.0 82.7