Hotspot

rpl2

IRa · 159600-161122 · 1523 bp

Back to Firmiana

Pi 0.000875

MarkerSeek score 31.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1523
variable_sites: 4
pi: 0.000875
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.3	55.4	1615 bp	1.0	40.2
rpl2_p2	ATCATTACGCTTCAACCGGG	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.3	55.4	1622 bp	1.0	40.2
rpl2_p3	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCT	50.0	30.4	58.3	54.3	1615 bp	1.0	40.2
rpl2_p4	ATCATTACGCTTCAACCGGG	AGACGAAGAAAGAAATTTTCTCT	50.0	30.4	58.3	54.3	1622 bp	1.0	40.2
rpl2_p5	ACGCTTCAACCGGGTTATTC	AGACGAAGAAAGAAATTTTCTCTC	50.0	33.3	58.6	55.4	1616 bp	1.0	40.2