Hotspot

petD

LSC · 82216-83436 · 1221 bp

Back to Firmiana

Pi 0.00328

MarkerSeek score 35.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1221
variable_sites: 12
pi: 0.00328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTCCTTACCTCGGGACCAA	CAGAATTGCCTCCCAGGATC	50.0	55.0	58.0	58.0	2058-2094 bp	1.0	81.3
petD_p2	GTGTTGACCGCATCTTTTGG	CAGAATTGCCTCCCAGGATC	50.0	55.0	57.9	58.0	2089-2125 bp	1.0	81.2
petD_p3	GTTGACCGCATCTTTTGGTG	CAGAATTGCCTCCCAGGATC	50.0	55.0	57.9	58.0	2087-2123 bp	1.0	81.2
petD_p4	GGTTACAGGCGTGGTTCTAG	CAGAATTGCCTCCCAGGATC	55.0	55.0	58.0	58.0	2128-2147 bp	0.833333	73.6
petD_p5	GGTTACAGGCGTGGTTCTAG	AGAATTGCCTCCCAGGATCT	55.0	50.0	58.0	58.1	2127-2146 bp	0.833333	73.0