Hotspot

clpP

LSC · 75482-77662 · 2181 bp

Back to Firmiana

Pi 0.00624

MarkerSeek score 45.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2181
variable_sites: 34
pi: 0.00624
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACCCTGTTAGTCCGTTCT	ATACGCAATGGGGGTTGATC	50.0	50.0	58.0	58.0	2935-2966 bp	1.0	83.1
clpP_p2	GTGAACGGAAGAACCCCATT	ATACGCAATGGGGGTTGATC	50.0	50.0	58.1	58.0	2721-2752 bp	1.0	82.6
clpP_p3	CTAGTACATGTTCCTCGGCG	ATACGCAATGGGGGTTGATC	55.0	50.0	57.9	58.0	2618-2648 bp	1.0	82.5
clpP_p4	AGTCGCACATACACCCTAGT	ATACGCAATGGGGGTTGATC	50.0	50.0	58.2	58.0	2633-2663 bp	1.0	82.2
clpP_p5	TGAACGGAAGAACCCCATTC	ATACGCAATGGGGGTTGATC	50.0	50.0	57.8	58.0	2720-2751 bp	1.0	81.8