Hotspot

trnN-GUU-trnR-ACG

IRa · 136153-136804 · 652 bp

Back to Firmiana

Pi 0.00286

MarkerSeek score 57.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 652
variable_sites: 5
pi: 0.00286
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	55.0	50.0	58.0	58.1	818-829 bp	1.0	81.4
trnN-GUU-trnR-ACG_p2	TGGGTTGAGAACGGGAATTG	AACAGATCCAGTGGAGACGA	50.0	50.0	58.1	58.1	859-870 bp	1.0	80.6
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	782-793 bp	1.0	80.4
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.8	58.1	782-793 bp	1.0	80.3
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	769-780 bp	1.0	80.3