Hotspot

rpl32-trnL-UAG

SSC · 119089-120199 · 1111 bp

Back to Firmiana

Pi 0.0054
MarkerSeek score 62.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1111
variable_sites
17
pi
0.0054
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTGAAAGCTTTTTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1176-1271 bp 1.0 83.7
rpl32-trnL-UAG_p2 CGGCGTTGAAAGCTTTTTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1177-1272 bp 1.0 83.7
rpl32-trnL-UAG_p3 CGGCGTTGAAAGCTTTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1186-1281 bp 1.0 82.8
rpl32-trnL-UAG_p4 CGGCGTTGAAAGCTTTTTCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.2 57.8 1185-1280 bp 1.0 82.2
rpl32-trnL-UAG_p5 CGGCGTTGAAAGCTTTTTCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.2 57.8 1184-1279 bp 1.0 82.2