Hotspot

rpl33-rps18

LSC · 73117-73535 · 419 bp

Back to Firmiana

Pi 0.0124

MarkerSeek score 43.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 15
pi: 0.0124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	GCAAACGCCTACGAAAAGAT	55.0	45.0	58.5	57.1	564-597 bp	1.0	72.3
rpl33-rps18_p2	CGACACAATACGCCTAGTCG	GGCAAACGCCTACGAAAAGA	55.0	50.0	58.5	59.1	565-598 bp	1.0	70.5
rpl33-rps18_p3	GACACAATACGCCTAGTCGA	GCAAACGCCTACGAAAAGAT	50.0	45.0	57.2	57.1	563-596 bp	1.0	69.9
rpl33-rps18_p4	TCGACACAATACGCCTAGTC	GCAAACGCCTACGAAAAGAT	50.0	45.0	57.2	57.1	565-598 bp	1.0	69.9
rpl33-rps18_p5	CGACACAATACGCCTAGTCG	GCAAACGCCTACGAAAAGATC	55.0	47.6	58.5	58.2	564-597 bp	1.0	69.7