Hotspot

psbZ-trnG-GCC

LSC · 37884-38698 · 815 bp

Back to Firmiana

Pi 0.0202

MarkerSeek score 65.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 44
pi: 0.0202
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAACCACTCAGCCATCTCTC	TTTGGTTTCGTTTCATCGCG	55.0	45.0	58.0	58.0	1576-1733 bp	1.0	86.1
psbZ-trnG-GCC_p2	CAACCACTCAGCCATCTCTC	CATATATGGAAGAGGGGCGC	55.0	55.0	58.0	58.0	1620-1777 bp	1.0	86.0
psbZ-trnG-GCC_p3	CAACCACTCAGCCATCTCTC	GATGATCATAGAGGGCCCCT	55.0	55.0	58.0	58.0	1675-1832 bp	1.0	85.9
psbZ-trnG-GCC_p4	GAGCTATCAACCACTCAGCC	TTTGGTTTCGTTTCATCGCG	55.0	45.0	58.1	58.0	1583-1740 bp	1.0	85.8
psbZ-trnG-GCC_p5	GGAGCTATCAACCACTCAGC	TTTGGTTTCGTTTCATCGCG	55.0	45.0	58.1	58.0	1584-1741 bp	1.0	85.8