Hotspot

ndhF

SSC · 110729-113020 · 2292 bp

Back to Filipendula

Pi 0.0109

MarkerSeek score 46.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2292
variable_sites: 86
pi: 0.0109
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTCGTTTTGAGAGGAAAATCG	ACCGCAACAAAGACGTTTTG	40.9	45.0	57.3	58.1	2852-2966 bp	0.8	55.4
ndhF_p2	TGTCGTTTTGAGAGGAAAATCG	CCGCAACAAAGACGTTTTGT	40.9	45.0	57.3	58.1	2851-2965 bp	0.8	55.4
ndhF_p3	GTCGTTTTGAGAGGAAAATCGA	ACCGCAACAAAGACGTTTTG	40.9	45.0	57.0	58.1	2851-2965 bp	0.8	53.4
ndhF_p4	GTCGTTTTGAGAGGAAAATCGA	CCGCAACAAAGACGTTTTGT	40.9	45.0	57.0	58.1	2850-2964 bp	0.8	53.4
ndhF_p5	TGTCGTTTTGAGAGGAAAATCG	AACCGCAACAAAGACGTTTT	40.9	40.0	57.3	57.4	2951-2967 bp	0.7	44.1