Hotspot

trnL-UAG-ccsA

SSC · 114311-114425 · 115 bp

Back to Filipendula

Pi 0.023
MarkerSeek score 64.50
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
115
variable_sites
7
pi
0.023
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT AGTCCTACGATTTCATCAACT 50.0 38.1 58.0 54.4 275-306 bp 1.0 50.6
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG AGTCCTACGATTTCATCAACT 50.0 38.1 58.0 54.4 274-305 bp 1.0 50.6
trnL-UAG-ccsA_p3 TAGACACGCTGCTCTTAGGA AGTCCTACGATTTCATCAACT 50.0 38.1 57.9 54.4 265-296 bp 1.0 49.8
trnL-UAG-ccsA_p4 CGAATAAGCCGCTATGGTGA AGTCCTACGATTTCATCAACT 50.0 38.1 57.9 54.4 291-322 bp 1.0 49.3
trnL-UAG-ccsA_p5 TCGAATAAGCCGCTATGGTG AGTCCTACGATTTCATCAACT 50.0 38.1 57.9 54.4 292-323 bp 1.0 49.3