Hotspot

rpl22-rps19

LSC · 82391-83001 · 611 bp

Back to Filipendula

Pi 0.0192

MarkerSeek score 68.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 48
pi: 0.0192
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACCAAGTCTGAAACCAAGCG	CCGACTCTAAATTTCCGGGG	50.0	55.0	58.7	58.1	759-789 bp	1.0	81.1
rpl22-rps19_p2	ACCAAGTCTGAAACCAAGCG	CGACTCTAAATTTCCGGGGG	50.0	55.0	58.7	58.1	758-788 bp	1.0	81.1
rpl22-rps19_p3	CCAAGTCTGAAACCAAGCGT	CCGACTCTAAATTTCCGGGG	50.0	55.0	58.7	58.1	758-788 bp	1.0	81.1
rpl22-rps19_p4	CCAAGTCTGAAACCAAGCGT	CGACTCTAAATTTCCGGGGG	50.0	55.0	58.7	58.1	757-787 bp	1.0	81.1
rpl22-rps19_p5	CCAAGTCTGAAACCAAGCGT	TCTAAATTTCCGGGGGCATG	50.0	50.0	58.7	57.9	753-774 bp	0.9	76.7