Hotspot

clpP-psbB

LSC · 71103-71857 · 755 bp

Back to Filipendula

Pi 0.0135

MarkerSeek score 65.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 37
pi: 0.0135
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GTGACGCTATCGACAGTCAT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.5	58.2	799-865 bp	1.0	79.5
clpP-psbB_p2	AGTGACGCTATCGACAGTCA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.5	58.2	800-866 bp	1.0	79.0
clpP-psbB_p3	GTGACGCTATCGACAGTCAT	AGCAATTAAACGACCCGGAT	50.0	45.0	57.5	57.6	842-908 bp	1.0	77.9
clpP-psbB_p4	GTGACGCTATCGACAGTCAT	ACACGATACCAAGGCAAACC	50.0	50.0	57.5	58.5	802-868 bp	1.0	77.7
clpP-psbB_p5	GTGACGCTATCGACAGTCAT	AAACGACCCGGATCATTCAA	50.0	45.0	57.5	57.5	835-901 bp	1.0	77.4