Hotspot

ycf3-psaA

LSC · 64202-64942 · 741 bp

Back to Filipendula

Pi 0.0151

MarkerSeek score 71.30

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 34
pi: 0.0151
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TGGAAACAAGCTATAGCGCT	GCCCATTCCTCGAAAGAAGT	45.0	50.0	57.3	57.9	881-946 bp	1.0	79.9
ycf3-psaA_p2	ATCAAGCCGCCGAGTATTG	GCCCATTCCTCGAAAGAAGT	52.6	50.0	58.0	57.9	898-963 bp	1.0	77.3
ycf3-psaA_p3	TGGAAACAAGCTATAGCGCT	TTTTACTTCTGGTTCCGGCG	45.0	50.0	57.3	58.5	834-899 bp	1.0	77.3
ycf3-psaA_p4	TGGAAACAAGCTATAGCGCT	TGTTCTTGAGAAATGGCCGG	45.0	50.0	57.3	58.5	906-971 bp	1.0	76.9
ycf3-psaA_p5	GATCAAGCCGCCGAGTATTG	GCCCATTCCTCGAAAGAAGT	55.0	50.0	59.1	57.9	899-964 bp	1.0	76.2